SARS-CoV-2 Nucleic Acid Detection Kit (RT-PCR)
【Test Principle】
This kit based on real-time PCR fluorescence technology, and the specific primers and corresponding fluorescent probes target to the conserved region of COVID-19 genome was designed for highly specific detection. By detecting the fluorescent signals changes, the COVID-19 nucleic acid from a throat swab or nasopharyngeal secretion specimen can be qualitatively detected.
【Sample Requirement】
1. Applicable sample type: Throat swab (Oropharyngeal swab/Nasal swab)
2. Sample collection
2.1 Sample time: The reagent is not affected by the specific clinical symptoms of patients, and
can be sampled at each stage of the course of the disease.
2.2 The test performance of the reagent is not affected when using the drugs described in the
analysis performance evaluation, and the sample can be taken normally. It is better to take
samples before using other unverified drugs
2.3 Throat swab requirement: It’s better to use long handle swab with polypropylene fiber head
or wood handle swab, please select the preservation solution suitable for cells and viruses.
It is better to directly select the commercial throat swab sampling tube.
3. Sampling method:
3.1 Oropharyngeal swab: Wipe both tonsils and the posterior pharyngeal wall with a swab with
moderate force to avoid touching the tongue.
3.2 Nasal swab: Use the swab to penetrate the nasal cavity for 2-3 cm, and rotate it for 2-3 times.
【Storage conditions and the period of Validity】
This kit shall be stored at the temperature below -20℃ in dark and avoid repeated freezing and
thawing (if freezing and thawing, must not exceed 4 times), with a validity period of 12 months. It
can be stored at -20℃ after opening the cover, with a validity period of 30 days. The kit shall be
transported in an ice box filled with 50% of the total volume of dry ice for no more than 5 days.
【Result judgment】
1. Determine the validity of the experiment:
The positive control FAM, VIC channel should have a typical amplification curve, and the Ct value is generally less than 36, but may
fluctuate due to different threshold settings of different instruments.
The negative control FAM, VIC channel should be non-amplified Ct. It is agreed that the above requirements must be met at the same time, otherwise this test is invalid.
2. Result judgment
FAM/VIC channel
Judgment result
Ct＜37
Sample test is positive
37≤Ct＜40
The amplification curve is S-shaped, and suspicious samples
need to be re-examined; if the re-examination results are
consistent, it is judged as positive, otherwise it is negative.
Ct≥40 Or No Amplification
Sample test is negative (or below the lower limit of kit detection)
Product Tags:
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SARS-CoV-2 RT PCR Test Kits Nucleic Acid Throat Swab Sample Fluorescence Channels Images
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